So far , achieved studies on calcium in embryo sac of angiosperm mostly located in memebrane - binding calcium and total calcium but little in free cytoplasmic calcium ( [ ca2 + ] cyt ) 到目前为止，对植物胚囊中钙的研究也绝大多数局限于总钙和膜钙的的测定，而对于被子植物胚囊内胞质游离钙的研究则几乎是空白。
They show gene activity characteristic of such cells ? and , with appropriate prodding , will differentiate into all of the basic cell types found in embryos , which themselves lead to the specialised cells of a complete human body 它们会表达其基因特征，只要加以特定的刺激，就会分化成胚胎里存在的所有种类细胞，并进一步特化为整个人体的全部细胞。
They show gene activity characteristic of such cells ? and , with appropriate prodding , will differentiate into all of the basic cell types found in embryos , which themselves lead to the specialised cells of a complete human body 它们表现出这些细胞的基因活动特征，只要施以恰当的刺激，这些干细胞能够分化为胚胎中所包含的所有基本细胞类型，在此基础之上，这些细胞将进一步发育成为人体所需的各种专能细胞。
Stem ce11 factor is a multipotent cytokine which is found in recent years , fol1owing research in vitro and in vivo found that scf and its receptors have familiar regu1ation function in mature brain with in embryo and developing period 干细胞因子是近年来发现的一种多功能细胞因子。近年来研究发现，干细胞因子（ stemcellfactor ， scf ）及其受体对胚胎期及成年后神经系统生长发育起调控作用，但其作用机制尚不明了。
Aei is active in a number of technology transfer projects providing expertise in embryo production and management , semen production , livestock management and production beef and dairy and has carried out a number of small ruminant sheep goats management and embryo projects 亚达国际出口有限公司在很多为胚胎生产和管理精子生产家畜管理和生产肉牛和奶牛提供技术的技术转让项目中都很积极，实施了很多小型反刍动物绵羊和山羊管理和胚胎项目。
( 2 ) there are few ideal techniques for mearsuring [ ca2 + ] cyt . in conclusion , both limitation delay studies of [ ca2 + ] cyt in embryo sac during fertilization . the thesis work is to investigate the connection of [ ca2 + ] cyt and fertilization by avoiding drawbacks noted above , say , employing an ideal experimental plant , torenia fournieri , with a naked embryo sac that protrudes from the micropyle 针对这种现状，本工作选用具有裸露胚囊的玄参科的蓝猪耳植物为研究材料，研究ca ~ ( 2 + )与受精过程中相关的细胞学事件的关系，探索[ ca ~ ( 2 + ) ] _ ( cyt )在被子植物双受精过程中的作用。
The research consist of four parts . the first part is multiplication , purification and electron microscope examination of the avian encephalomyelitis virus . a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos . respectively . after incubation for 10 days , the urinay vesicle liquid was collected . making a comparison the size of the chickens embryos between the test group and the control group , the results showed that the size of the control group is bigger than that of the test group . purified virions were examined under the electron microscope , the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos . the second part was seguence analysis of the genome of the aev - nh937 . nine pairs of primers were designed according to published calnek vaccine strain of aev 本研究共分四个部分：第一部分为aev的增殖，纯化和电镜观察，用1 : 5倍稀释的aev - nh937株和阴性对照pbs分别经卵黄囊接种于6dspf鸡胚，继续孵化10d后，收集尿囊液。比较接种组和健康对照组鸡胚的大小，结果显示，健康对照组鸡胚明显大于接种组。分离、提纯aev ，把纯化的病毒在电镜下观察，证明确有大量aev病毒粒子存在，说明aev在鸡胚中成功扩增；第二部分是aev - nh937基因组的序列测定工作。
The stage when the block occurs has been correlated to the time the embryonic genome takes over control of development from the maternal genome , which occurs in mouse at the 2 - cell stage . the embryos of balb / cxicr fl mouse show 2 - cell block in vitro and the effects of several culture media on overcoming the 2 - cell block in embryos development were studied . m16 ( sigma ) , in which 96 . 2 % of 1 - cell embryos developed to 4 - cell stage , were shown to be effective in overcoming the 2 - cell block 培养5h后， 53去核卵母细胞可见标记的mtocs ，但数量明显少于对照组，而又多于培养0h的去核细胞，说明此时53的化学去核卵胞质中确有mtocs的存在，其微管聚合能力正在恢复； mtocs标记，但其辐射产生的微管纤维短，结构还不够丰满；其余细胞的微管蛋白则丧失重新装配能力。
During male gametogenesis , the spermatogonia became primary spermatozoa through mitosis , and then became fourth equivalent cytoplasm , round sperm through meiosis . after maturation , they participated in embryo development with the mature oocyte through fertilization . but in female gametogenesis , the primary oocyte formed a matured egg and 1 ~ 3 polar bodies ( pb ) 雄性动物精子发生过程中精原细胞最后一次有丝分裂形成二倍体精母细胞后，经过两次减数分裂形成了四个含等量细胞质、圆形的精子细胞，最终发育成四个具有正常生理功能的精子，与成熟卵母细胞受精后参与胚胎发育。
Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body 将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。
